Laboratory of Chromatography and Mass Spectrometry | Jagiellońskie Centrum Innowacji

Laboratory of Chromatography and Mass Spectrometry

Chromatographic analysis


Chromatographic analyses are among the most commonly used research techniques related to separation of complex mixtures to individual components and their quantitative assaying. A technique of substance separation is based on passing the studied mixture through a column filled with an appropriate stationary bed and separating the mixture components by creating an equilibrium of each analyte between the stationary and the mobile phase.


Chromatographic analyses are widely used in quality control in industry and they are utilized in different ways, including:

  • Raw material analysis (control of semi-product identity, chemical analysis of contaminations)
  • Testing of ready product (control of product identity, contamination analysis)
  • Purification of chemical substances


The Laboratory of Chromatography and Mass Spectrometry of the Jagiellonian Center of Innovation also performs analytical and consulting services within the scope of qualitative and quantitative analysis of compounds using liquid chromatography (HPLC, UHPLC), gas chromatography (GC and GC-MS), and supercritical state chromatography (CO2) (SFC).


We offer:

  • Quantitative analyses of mixtures of liquid organic compounds (GC, UHPLC)
  • Purity analyses of organic compounds (GC, GC-MS, UHPLC)
  • Quality analyses of organic compounds (GC, GC-MS)

Liquid chromatography

The Laboratory of Chromatography and Mass Spectrometry of the Jagiellonian Center of Innovation is equipped with analytic UHPLC systems (Dionex) and kits for preparative purification of chemical substances combined with the following detectors:

  • UV – VIS “DAD” (190–900 nm), ELSD
  • Corona Veo RS (working temperature range +5°C to +100°C)
  • MALS-QELS (ability to determine masses from 100 Da to 1250 kDa)
  • Q-ToF – (MS),(ESI, APCI) (spectral range from 20 to 15000 m/z)
  • Fluorescence FLD (spectral range: Ex from 200 to 630; Em 220 to 650)
  • Refractometric RI
  • Preparative LC-MS kit (Shimadzu) with detectors: UV (from 190 to 370nm), ELSD and MS (“single quadrupole”)


The equipment allows:

  • broad range of analyses
  • significantly shorter analysis time by the use of UHPLC systems,
  • detection of substances invisible in UV-VIS range with use of

Corona/ELSD detectors

  • high-yield purification of chemical substances

Gas chromatography


  • chromatography sets (Shimadzu) with an autosampler for injection of

liquid samples and HeadSpace accessories

  • detectors: MS detector and flame ionization detector FID,


The equipment enables:

  • analyzing volatile substances collected from over the headspace owing to

the use of HeadSpace accessory

  • qualitative and quantitative analysis of unknown substances owing to a built-in mass library

Chromatography in supercritical state (CO2) (SFC)

  • chromatographic sets (Waters) with an analytical system
  • PDA detectors for optimization of separation methods
  • fully automated preparative set combined with detectors: PDA (from 190 to 900 nm) and MS (“single quadrupole”)


The equipment allows:

  • significant shortening of time of chiral compound analysis
  • separation of chiral substances that cannot be separated using standard chromatography methods (LC) due to their characteristics (high polarity, lability).
  • separation of optically active compounds with a set of chromatography columns

Head of the Laboratory of Chromatography and Mass Spectrometry
Paweł Hrynczyszyn, PhD


Holder of PhD in chemistry with a broad experience in chemical analytics. He completed PhD studies at the Faculty of Chemistry of the Nicolaus Copernicus University in Toruń, where he dealt with the application of nuclear magnetic resonance (NMR) technique in quantitative analysis. He gained his professional experience e.g. at the Institute of Organic Chemistry in the Technical University in Łódź. He has collaborated with the Jagiellonian Center of Innovation since 2015 and is responsible for projects with chromatographic methods of qualitative and quantitative analysis.


Anna Jonda – Chemical analysis specialist
Dominika Szot-Przewrocka – Analytical chemist
Monika Strzałkowska – Junior chemical analysis specialist


For a quote, please contact:

Telephone: +48 12 297 46 12

Mobile: +48 512 785 197

Our clients

Application notes

Anna Jakubowska, Jorg Pabel, Marek Żylewski, Klaus T. Wanner, Katarzyna Kulig

Asymmetric synthesis of all four stereoisomers of 1-amino-3-hydroxy-cyclopentane-1-carboxylic acid

Waldemar Tejchman, Izabela Korona-Głowniak, Anna Malm, Marek Żylewski, Piotr Suder

Antibacterial properties of 5-substituted derivatives of rhodanine- 3-carboxyalkyl acids

Waldemar Tejchman, Ewa Żesławska, Krzysztof Zborowski, Wojciech Nitek, Marek Żylewski

The synthesis, molecular structure and spectra properties of sulfur and selenium deferiprone analogues


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