Microbiological Laboratory

The Microbiological Laboratory of the Jagiellonian Center of Innovation performs microbiological analyses of biological, pharmaceutical and environmental materials. Diagnostics is performed using a classical method (smear to a medium, growth, microscopic assessment) and innovative diagnostic method using MALDI-TOF mass spectrometry.

 

We offer:

  • Testing of microbiological purity of cosmetics as per the following standards:
    PN-EN ISO 21149:2017-07 “Counting and detection of mesophilic aerobic bacteria”
    PN-EN ISO 16212:2017-08 “Determination of yeast and mold number”
    PN-EN ISO 21150:2016-01 “Detection of Escherichia coli presence”
    PN-EN ISO 22718:2016-01 “Detection of Staphylococcus aureus presence”
    PN-EN ISO 22717:2016-01 “Detection of Pseudomonas aeruginosa presence”
    PN-EN ISO 18416:2016-01  “Detection of Candida albicans presence”
  • Analysis of purity of microbiological water purified as per pharmacopoeial method (Polish Pharmacopeia X)
  • Assessment of efficiency of antimicrobial protection of a cosmetic product, as per PN-EN ISO 11930:2012 standard
  • Microbiological monitoring of the production environment of cosmetics, food, and pharmaceuticals.

 

 

Identification of microorganisms using MALDI-TOF mass spectrometer

 

The Microbiological Laboratory of the Jagiellonian Center of Innovation offers identification of bacteria, yeast-like fungi, filamentous fungi and dermatophytes. The analysis is performed using MALDI-TOF mass spectrometry with the use of laser desorption/ionization supported with a matrix with time-of-flight analyzer, using a MALDI-TOF mass spectrometer Microflex LT from Bruker.

 

Identification of a microbe is performed within a few minutes based on the unique protein profile of the microorganism (ribosomal proteins), the so-called molecular “fingerprint”, which saves a lot of time as compared to 24–48 hours needed to identify a microbe using biochemical methods.

 

Comprehensive, continuously updated database of about 3,000 species allows us to identify even microorganisms with weak biochemical properties.

 

Before placing you identification of microorganisms order, contact us to determine the study conditions and identification option.

OPTION I – colony analysis (FROM 35 PLN + VAT)

Culture a colony using any medium and send it via courier to Krakow. We will perform fast identification using MALDI-TOF, and provide a result report by e-mail.

 

  • Grow the colonies.*
  • Protect the material in an appropriate way.
  • Download the order form from JCI’s website, fill it in, and attach it to your shipment.
  • Notify JCI that the specimen will be shipped.
  • Send your shipment to JCI.
  • Get your results by e-mail.

 

The price for identification of one bacterial or fungal species is from 35 PLN net + 23% VAT.

 

How to prepare a colony for identification:

  • The microorganisms can be delivered to JCI in any medium (differences in culture conditions have little influence on identification).
  • A single colony is enough to perform identification.
  • The culture should be clean, about 18-24 hours.
  • In case of microbes with slow growth, a few-day culture is recommended.
  • Microbes on culture plates prepared for MALDI-TOF identification should be kept at room temperature.
  • Microbes on culture plates prepared for MALDI-TOF identification cannot be kept refrigerated.

OPTION II – sample analysis (FROM 45 PLN + VAT)

Prepare the material (product sample or smear secured on a transfer medium) to be tested and send it via shipping company to Krakow. We will grow the colonies, perform fast identification using MALDI-TOF, and provide a result report by e-mail.

 

  • Prepare the material, secure the sample and prepare it for shipment.
  • Download the order form from JCI’s website, fill it in, and attach it to your shipment.
  • Notify JCI that the specimen will be shipped.
  • Send your shipment to JCI.
  • Get your results by e-mail.

 

The price for identification of one bacterial or fungal species is from 45 PLN net + 23% VAT.

Discount policy

Please read our discount policy which depends on the numbers of samples you send to us:

  • 5% discount when you send 100–199 samples per month
  • 10% discount when you send 200–299 samples per month
  • 15% discount when the number of samples is over 300 in a month.

Form for submitting samples for analysis

Before you send your samples to us for analysis, please print and fill in the application form, and attach it to your shipment.

 

Download your application form >>

Head of the Microbiological Laboratory
Małgorzata Głowacka-Twardokęs 

 

She graduated with a major in Biology at the Faculty of Biology and Earth Sciences of the Jagiellonian University and post-degree studies in Medical Analytics at the Faculty of Pharmacy of the Jagiellonian University (title: laboratory diagnostician). She gained experience working in clinical microbiology laboratories e.g. in the University Children’s Hospital in Krakow and in a Regional Hospital named after Blessed Marta Wiecka in Bochnia. In the Jagiellonian Center of Innovation, she is responsible for the work and research in the Microbiological Laboratory.

 

Team:

Kamila Nawieśniak – Junior microbiology specialist

 

For a quote, please contact: 

Telephone: +48 12 297 46 12

Mobile: +48 512 785 197
E-mail: agata.mleczkowska@jci.pl

Our clients

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