The Microbiology Laboratory of the Jagiellonian Centre of Innovation offers identification of bacteria, yeast-like fungi, filamentous fungi and dermatophytes. Analysis is carried out by MALDI-TOF mass spectrometry using laser desorption/ionisation, matrix-assisted with a time-of-flight analyser using a MALDI-TOF Microflex LT mass spectrometer from Bruker.

 

Identification of the micro-organism is performed in the course of a few minutes, based on the micro-organism’s unique protein profile (ribosomal proteins), a so-called molecular ‘fingerprint’, which is a great time-saver compared to the 24-48 hours needed to identify micro-organisms by biochemical methods.

 

This is of great importance for clinical samples. In addition, the use of MALDI-TOF mass spectrometry enables rapid identification of anaerobic microorganisms and those that cannot be identified by biochemical methods.

 

Our database is regularly updated and currently allows the identification of approximately 5,000 species.

 

Before ordering microbial identification, contact us to establish the test conditions and identification option.

 

 

Sample order form for testing

Before sending samples to us for analysis, please print and complete the order form and enclose it with your shipment.

 

 

 

Option I – Colony analysis (46 PLN net)

Grow colonies on any medium and send it by courier to Krakow. We will carry out a rapid identification for you using MALDI-TOF and you will receive a report with the results by e-mail.

 

• Grow colonies. *
• Secure the material accordingly.
• Download the order form from the JCI website, fill it in and attach it to the parcel.
• Notify JCI that the sample will be sent.
• Send to JCI (to JCI Microbiology Laboratory, Building B, 14 M. Bobrzyńskiego Street, 30-348 Cracow).
• Receive an email with the results.

 

The price for the determination of one bacterial or fungal species is PLN 46 net + 23% VAT

 

*Preparation of colonies for identification:

• The micro-organisms supplied can be grown on any medium.
• A single colony (obtained by surface culture) is already sufficient for testing. In the case of very fine colonies, the bacteria need to be multiplied in larger numbers.
• The culture should be clean and its time should not exceed 48h. This is particularly important for the identification of Bacillus, as the degree of sporulation (related to the age of the culture) affects the quality of the spectrum obtained.
• For microorganisms with slow growth (e.g. anaerobic bacteria), a culture of 48 – 96h is acceptable.
• If older cultures are received, the laboratory can re-passage the micro-organism on request (this does not apply to anaerobic bacteria), at an additional cost.
• Microorganisms on culture plates prepared for identification with MALDI-TOF should be stored at room temperature (samples stored at reduced temperature should be re-passaged!).

 

We would very much appreciate it if you would complete the order form carefully in order to provide the best possible service (survey).

 

 

OPTION II – analysis from a sample (individual pricing)

 

Analysis from a sample is possible ONLY after prior telephone consultation with the Laboratory Manager (tel. 12 340 21 27).

 

Prepare the material (product sample or swab protected on transport medium) to be tested and send it by courier to Krakow. We will grow the colonies, carry out a rapid identification for you using MALDI-TOF, and you will receive a report with the results by e-mail.

 

• Prepare the material, secure the sample and prepare to send.
• Download the order form from the JCI website, fill it in and attach it to the parcel.
• Notify JCI that the sample will be sent.
• Send to JCI (to JCI Microbiology Laboratory, Building B, 14 M. Bobrzyńskiego Street, 30-348 Cracow).
• Receive an email with the results.

 

Discount policy*:

 

Take a look at our discount policy, which depends on the number of samples sent to us:

• 5% discount for 100 – 199 samples per month
• 10% discount for 200 – 299 samples per month
• 15% discount when the number of samples exceeds 300 in a month.

 

*Discount policy does not apply to subscription services

 

 

 

We offer :

• microbiological purity testing of cosmetics (according to ISO standards, Polish Pharmacopoeia X)
• testing of antiseptics (Polish Pharmacopoeia X)
• food testing (ISO, non-accredited testing)
• water testing (ISO, non-accredited testing)
• production purity testing
• environmental research

Cosmetics and chemical products

• PN-EN ISO 21148:2017-07 Cosmetics – Microbiology – General instructions for microbiological examination
• PN-EN ISO 17516:2014-11 Cosmetics – Microbiology – Microbiological limits
• PN-EN ISO 29621:2017-04 Cosmetics – Microbiology – Guidelines for the risk assessment and identification of microbiologically low-risk products
• PN-EN ISO 21149:2017-07 Cosmetics – Microbiology – Enumeration and detection of aerobic mesophilic bacteria
• PN-EN ISO 16212:2017-08 Cosmetics – Microbiology – Enumeration of yeast and mould
• PN-EN ISO 18416:2016-01 Cosmetics – Microbiology – Detection of Candida albicans
• PN-EN ISO 21150:2016-01 Cosmetics – Microbiology – Detection of Escherichia coli
• PN-EN ISO 22717:2016-01 Cosmetics – Microbiology – Detection of Pseudomonas aeruginosa
• PN-EN ISO 22718:2016-01 Cosmetics – Microbiology – Detection of Staphylococcus aureus
• PN-EN ISO 11930:2019-03 Cosmetics – Microbiology – Evaluation of the antimicrobial protection of a cosmetic product
• PN-EN ISO 18415:2017-07 Cosmetics – Microbiology – Detection of specified and non-specified microorganisms

 

Food and animal feeding stuffs

• PN-EN ISO 4833-1:2013-12 AP1:2016-11 Microbiology of the food chain – Horizontal method for the enumeration of microorganisms – Part 1: Colony count at 30°C by the pour plate technique
• PN-EN ISO 4833-2:2013-12 AC 2014-04 Microbiology of the food chain – Horizontal method for the enumeration of microorganisms – Part 2: Colony count at 30°C by the surface plating technique
• PN-ISO 7954:1999 Microbiology – General guidance for enumeration of yeasts and moulds – Colony count technique at 25°C
• PN-ISO 21527-1:2009 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of yeasts and moulds – Part 1: Colony count technique in products with water activity greater than 0,95
• PN-ISO 21527-2:2009 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of yeasts and moulds – Part 2: Colony count technique in products with water activity less than or equal to 0,95
• PN-ISO 16649-2:2004 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli – Part 2: Colony-count technique at 44°C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide
• PN-EN ISO 21528-1:2017-08 Microbiology of the food chain – Horizontal method for the detection and enumeration of Enterobacteriaceae – Part 1: Detection of Enterobacteriaceae
• PN-EN ISO 21528-2:2017-08 Microbiology of the food chain – Horizontal method for the detection and enumeration of Enterobacteriaceae – Part 2: Colony-count technique
• PN-EN ISO 6888-2:2001/A1:2004 Microbiology of the food chain – Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) – Part 2: Method using rabbit plasma fibrinogen agar medium
• PN-ISO 4832:2007 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of coliforms – Colony-count technique
• PN-EN ISO 6579-1:2017-04 Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Detection of Salmonella spp.
• PN-EN ISO 11290-1:2017-07 Microbiology of the food chain – Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Part 1: Detection method
• PN-EN ISO 11290-2:2017-07 Microbiology of the food chain – Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Part 2: Enumeration method
• PN-EN ISO 7932:2005 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of presumptive Bacillus cereus – Colony-count technique at 30°C
• PN-EN ISO 7937:2005 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of Clostridium perfringens – Colony-count technique
• PN-ISO 15213:2005 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions
• PN-ISO 7889:2007 Yogurt – Enumeration of characteristic microorganisms – Colony-count technique at 37°C
• PN-ISO 29981:2012 Milk products – Enumeration of presumptive bifidobacteria – Colony count technique at 37°C
• PN-ISO 15214:2002 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of mesophilic lactic acid bacteria – Colony-count technique at 30°C
• PKN-ISO/TS 11059:2009 Milk and milk products – Method for the enumeration of Pseudomonas spp.

 

Water

• PN-EN ISO 8199:2019-01 Water quality – General requirements and guidance for microbiological examinations by culture
• PN-EN ISO 6222:2004 Water quality – Enumeration of culturable micro-organisms – Colony count by inoculation in a nutrient agar culture medium
• PN-EN ISO 9308-1:2014-12/A1:2017-04 Water quality – Enumeration of Escherichia coli and coliform bacteria – Part 1: Membrane filtration method for waters with low bacterial background flora
• PN-EN ISO 11731:2017-08 Water quality – Enumeration of Legionella
• PN-EN ISO 7899-2:2004 Water quality – Detection and enumeration of intestinal enterococci – Part 2: Membrane filtration method
• PN-EN ISO 14189:2016-10 Water quality – Enumeration of Clostridium perfringens – Method using membrane filtration
• PN-EN 26461-2:2001 Water quality – Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) – Part 2: Method by membrane filtration
• PN-EN ISO 16266:2009 Water quality – Detection and enumeration of Pseudomonas aeruginosa – Method by membrane filtration

 

Plastics

• PN-EN ISO 846:2002 Plastics – Evaluation of the action of microorganisms
• ISO 22196:2011/JIS Z 2801 Measurement of antibacterial activity on plastics and other non-porous surfaces
• PN-EN ISO 20743:2013-10 Textiles – Determination of antibacterial activity of textile products
• ISO/DIS 8124-12 Safety of toys – Part 12: Microbiological Safety

 

Pharmacopoeial methods:

Microbiological purity test:

• Microbiological examination of non-sterile products (Total viable aerobic count) –  Polish Pharmacopoeia XII (FP) monograph no. 2.6.12
• Microbiological examination of non-sterile products (Test for specified micro-organisms) –  Polish Pharmacopoeia XII (FP) monograph no. 2.6.13

 

Efficacy of antimicrobial preservation:

• PET/Challenge test – Polish Pharmacopoeia XII (FP) monograph no. 5.1.3.

We also carry out non-standard studies on request. In consultation with the client, we flexibly tailor experience designs to meet specific needs.

 

Innovative research e.g:

• testing the effectiveness of UVC lamps, checking the level of microbial reduction
• microbiological testing of files, fabrics and coatings

To meet your expectations, we have created a subscription offer for analyses in the Microbiology Laboratory. We offer 3 different packages that combine the following advantages:

 

• lower prices depending on the choice of subscription package
• short turnaround time – 12h from sample delivery to JCI
• report in electronic form
• subscription invoice issued at the beginning of the month.

 

Package types:

 

 

 

The subscription contract can be concluded and paid for in advance for the entire year. The annual subscription is then reduced by an additional 5% of the total amount! 

 

*Standard price for testing 1 sample is 46 PLN net

 

Delivery of samples:

• inform the JCI no later than 24 hours before the scheduled delivery date of the samples, together with an indication of the quantities and types of analyses to be commissioned, as well as the specific storage conditions of the samples
• an order form must be submitted with the sample
• samples should be delivered on working days, between 7:00 a.m. and 4:00 p.m.

 

Analysis of the report:

The analysis will be carried out in time:

• up to 12 hours after acceptance of sample for bacterial analysis
• up to 72 hours after acceptance of the sample for fungal analysis

 

not including Saturdays or public holidays.

 

The report, in the form of a PDF, will be drafted and delivered after the analysis.