Accelerating Discovery with Reliable, High-Quality Proteins

The Protein Production Laboratory provides comprehensive protein science services designed to advance early-stage drug development and biotechnology research.

Through expertise in molecular biology, protein engineering, and biophysical analysis, the laboratory delivers high-quality recombinant proteins optimized for discovery assays, screening campaigns, and structural studies.

Comprehensive Protein Production

The laboratory offers a fully integrated workflow for recombinant protein production, tailored to the specific properties of each target and its downstream application.

Capabilities include:

• Construct and tag design – Strategic construct planning and tag selection for optimal yield, solubility, and purification efficiency.
• Expression optimization and scale-up – Evaluation of test expression and production at preparative scales, adapted to the target’s characteristics.
• Purification strategies – Customized purification of diverse protein classes, including kinases, nucleic acid interactors, molecular complexes, single domains, and engineered variants.
• Rigorous quality control – Analytical validation to ensure protein purity, homogeneity, stability, and functional integrity.

Analytical Quality Control

Each produced protein undergoes detailed quality assessment to guarantee reproducibility and data reliability.
The scope and depth of quality control are adapted to project requirements and downstream applications, ensuring that every protein is characterized according to its intended use in research, screening, or structural studies.

Purity and Concentration

• SDS-PAGE and spectrophotometric analyses

Homogeneity and Oligomeric State

• Analytical size-exclusion chromatography (HPLC-based SEC) and SEC-MALS

Stability and Folding

• Thermal stability profiling
• Circular dichroism (CD) for secondary structure and folding confirmation

Protein Identity

• Mass spectrometry (intact mass and peptide mapping)
• SEC-MALS

Biophysical Characterization and Interaction Analysis

In addition to production, the laboratory provides in-depth protein characterization services supporting the optimization of lead molecules, formulation stability, and mechanistic studies.

• Small Molecules Screening
  Comprehensive screening services supporting all stages of drug discovery, including hit identification, hit validation, and hit-to-lead optimization. A combination of biophysical and biochemical methods—such as Surface Plasmon Resonance (SPR), Microscale Thermophoresis (MST), and thermal shift profiling—is employed to detect and characterize small molecule interactions with target proteins. Complementary assays enable rapid and quantitative assessment of binding kinetics, affinities, and mechanisms of interaction, facilitating the selection and optimization of promising lead compounds.
• Protein–ligand and protein–protein interactions
  Binding kinetics and affinities determined using MST, SPR, and complementary methods.
• Protein–nucleic acid interactions
  Quantitative assessment of binding parameters and specificity.

• Stability analysis
  Detection of stability changes upon ligand or partner binding using thermal stability profiling

• Structural profiling
  Circular dichroism (CD) provides insights into protein folding and conformational changes, supporting structural biology and formulation research.

Why Partner with Us

• Integrated expertise – From construct design to analytical validation, every stage is managed by experienced scientists.
• Results backed by analytical rigor – Comprehensive QC ensures reproducibility and reliability in downstream applications.
• Flexible collaboration – Adapted workflows to specific project’s goals, timelines, and assay requirements.

For a quote, please contact:

Mobile: +48 517 917 834
E-mail: sales@jci.pl