Tissue and Cell Laboratory
Cell cultures are grown in the Laboratory for Tissue and Cell Growth of the Jagiellonian Center of Innovation, in rooms with high level of air purity (cleanrooms). The cultures are performed according to the GMP. The premises are adapted to growing human tissues and cells for clinical use.
Constant monitoring of parameters within the rooms and of laboratory equipment while maintaining sterile working conditions is the key to safe work with materials of human origin, considering both, the biological material prepared for transplantation, and the occupational safety of the staff. This results in a possibility to treat patients using the latest techniques and the most efficient treatment methods available, including the procedure of transplantation of autologous cells grown in vitro. The Tissue Bank allows for keeping the material to ensure adequate amount of allogeneic material necessary for transplantations, e.g. in the case when the procedure must be repeated.
The Laboratory for Tissue and Cell Growth offers cooperation within the scope of:
- Isolation and growth of stem cells from heterogeneous populations, including those from bone marrow, umbilical cord blood, Wharton’s jelly, mobilized peripheral blood, and other mature tissues
- Preparation of the obtained cells for autologous applications
- Banking and storing cells
Cell tests are performed using eukaryotic cells. The most standard methods refer to cytotoxicity of external, physical and chemical factors on the cell biology. To obtain this goal, the following tests are performed: MTT, MTS, and LDH after choosing the appropriate cellular model and based on the recommended standards.
The MTT and MTS tests allow for the measurement of mitochondrial enzyme activity in the cell. Oxidative activity of mitochondria is directly proportional to the amount of yellow-colored tetrazole MMT or MTS salt reduced to formazan (blue), as measured spectrophotometrically. The difference between the tests is that MTT, in contrast to MTS, results in an insoluble product that has to be suspended in an organic solvent. In precisely defined experimental conditions, these tests may help determine the viability of non-dividing, yet metabolically active cells.
Lactic dehydrogenase (LDH) is an intracellular enzyme. Mechanical damage to plasmatic membrane and cell death caused by external factors result in release of LDH from the cells to the environment. Assaying the LDH activity in supernatant is a measure of toxicity of the tested substance against the cultured cells. Enzymatic reactions described in this method occur at two stages, resulting in a colored product that can be measured spectrophotometrically.