Analysis of Structure and Formulation of Medication

The structural analysis allows for determining spatial and constitutional form of chemical compounds. This can be achieved using a few complementary techniques, including nuclear magnetic resonance, VIS, UV and IR spectroscopy, as well as mass spectroscopy.

The Jagiellonian Center of Innovation offers measurements using the above mentioned techniques, with high-quality instruments.

NMR Spectroskopy

The NMR Spectroskopy Laboratory of the Jagiellonian Center of Innovation has Avance III HD 400 MHz (Bruker) spectrometer equipped with gradient probes working in a normal (BBO) and inverse detection (BBI). Both probes allow for measuring 1H NMR spectra and spectra of other nuclei (wide-range probes).

The instruments enable:

  • identification of chemical substances along with the determination of their chemical structure, and often also spatial structure and compound purity
  • measurement in the temperature range from -150 to + 150ºC
  • measurement of 1D and 2D NMR spectra, including specialised correlation measurements (NOE, DEPT, COSY, NOESY, HSQC, HMBC)

IR Spectroscopy

A laboratory in the Jagiellonian Center of Innovation has a FTIR Thermo Scientific Nicolet iS10 spectrometer equipped with ATR attachment. The measurements can be performed in two modes: transmission (KBr tablets or in nujol) and ATR (Attenuated Total Reflection) mode.

The instruments allow for:

  • verification of active substances and excipients in pharmaceuticals
  • identification of polymers, co-polymers and additions in plastics, as well as analysis of volatile liquids
  • measurements without initial preparation of the samples (ATR mode)
  • measurement of both solid and liquid form

HRMS Spectroskopy

A laboratory of the Jagiellonian Center of Innovation has a QTOF Impact II (Bruker) mass spectrometer offering the option of direct injection as well as connection with a liquid uHPLC chromatograph which makes it possible to separate the analyzed mixture. In the instrument, the following ion sources can be used: ESI for large molecular weight compounds (e.g. proteins) and APCI for analysis of polar and moderately polar compounds of small molecular weight (e.g. alkaloids).

The instruments enable:

  • broad range of mass analyses (two ionization sources – ESI, APCI)
  • detailed fragmentation analysis (MS/MS double quadrupole)
  • mass detection range from 20 to 15 000 m/z with resolution 10-6 and sensitivity 0.1 ng/mL.

UV-VIS Spectroscopy

A laboratory of the Jagiellonian Center of Innovation has a UV-VIS spectrophotometer (Agilent Technologies Cary 60).

UV-VIS measurements allow for:

  • monitoring of chemical and biological reactions kinetics
  • recording absorption spectra
  • measuring absorbance profile in the range from 190 nm to 1100 nm
  • assessment of raw material as well as end-product quality
  • analysis of photochemical reaction in-situ during light exposure of the sample

The instrument is equipped with an optical fiber probe for direct measurement of absorbance in a container different than a cuvette.

Analysis of Medication Formulation

The Jagiellonian Center of Innovation performs pharmaceutical analyses related to the monitoring of formulation methods for the designed as well as ready medications. A laboratory of the Jagiellonian Center of Innovation has automated instruments as per pharmacopoeia recommendations, which enable the measurement of:

  • release time of active substance from solid and lipophilic formulations
  • disintegration time of tablets, suppositories and globules
  • total deformation time for lipophilic suppositories
  • crush resistance (with simultaneous monitoring of thickness and diameter)
  • abrasion resistance

Stability tests and tests of conformance with packaging can be also performed using stability chambers. Further qualitative and quantitative measurements are also possible since the laboratory is equipped with complex analytical instruments.